Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mecp2

Cell type

Cell type Class
Neural
Cell type
Cerebellar Cortex
MeSH Description
The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Attributes by original data submitter

Sample

source_name
Cortex
genotype
wild type
strain
C57BL/6
chip antibody
Total MeCP2 antibody (Chen et al. 2003)
age
8 weeks

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
total MeCP2 ChIP was performed as described in Cohen et al. Neuron 2012, Ebert et al Nature 2013 Single-end short read sequencing libraires were prepared using standard protocols, following the manufacturers instructions. Libraries were sequenced on the HiSeq 2500

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
21695345
Reads aligned (%)
95.4
Duplicates removed (%)
15.6
Number of peaks
350 (qval < 1E-05)

mm9

Number of total reads
21695345
Reads aligned (%)
95.1
Duplicates removed (%)
15.6
Number of peaks
395 (qval < 1E-05)

Base call quality data from DBCLS SRA