Antigen

Antigen Class

TFs and others

Antigen

Mecp2

Cell type

Cell type Class

Neural

Cell type

Cerebellar Cortex

MeSH Description

The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Sample

source_name

Cortex

genotype

wild type

strain

C57BL/6

chip antibody

Total MeCP2 antibody (Chen et al. 2003)

age

8 weeks

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

total MeCP2 ChIP was performed as described in Cohen et al. Neuron 2012, Ebert et al Nature 2013 Single-end short read sequencing libraires were prepared using standard protocols, following the manufacturers instructions. Libraries were sequenced on the HiSeq 2500

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

21695345

Reads aligned (%)

95.4

Duplicates removed (%)

15.6

Number of peaks

350 (qval < 1E-05)

mm9

Number of total reads

21695345

Reads aligned (%)

95.1

Duplicates removed (%)

15.6

Number of peaks

395 (qval < 1E-05)